Yes, absolutely. There is actually a reference thread for this type of thing in JFO here:
http://www.survivinginfidelity.com/forums.asp?tid=510509
In your particular case, this is what I would do:
Immediately pull 50% off all joint funds and put them in a new account in your name only. Inform her after the fact, so she doesn't take ALL the money before you can. Change your direct deposit to the new account, but be prepared to put half of it back in the joint account if legally advised to do so.
If you do not already have access to all the household bills, get them ASAP. Put them all in Mint or Quicken, or set them up in your bank's bill pay system, so you have a one-stop place to view them all and be able to show they are being paid. You may need to figure out if certain bills are on auto-pay in the joint account and change that if needed.
It would be ideal for you to put all the household bills in your name if you plan to stay in the residence. Otherwise, WS could decide to leave, and cancel all the accounts without telling you.
See a L ASAP. Find out if you need to pay for your household bills out of the joint account, or if you can do it out of your new account. Find out all the financial options and logistics of D.
With the blessing of your L, tell your WS how you plan to pay for household expenses and joint debts but not debts or expenses in her name only, and of any household/joint bills you expect her to pay if applicable. If she wants to be reimbursed for other things, she can 1)submit receipts and 2) give you statements on her credit cards going back 12 months (if you don't already have access to this). You will need these statements anyway for D, and it will likely provide you more proof of the A if that is an advantage for you in your state.
Seeing a L ASAP is critical. You want to do this by the book, and it varies so much by state/county.
BS 43, SAWH 38. M 15years, together 17. Body count in the triple digits. Both in recovery, trying to R.
Three kids under age 11.